Influence of honey in mice bearing Ehrlich carcinoma on immune status

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Influence of honey in mice bearing Ehrlich
carcinoma on immune status
Ahmed G. Hegazi Eman H. Abdel- Rahman Fayrouz
Abd Allah and Amr M. Abdou National Research
Center, Dokki, Giza, Egypt ahmed_at_ahmedhegazi com
and ahmedhegazi128_at_gmail.com
www.ahmedhegazi.com
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3rd International Conference and Exhibition on
Clinical Cellular Immunology September 29 -
October 01, 2014 Baltimore, USA

• Influence of honey in mice bearing Ehrlich
  carcinoma on immune status

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Introduction

• Cancer continues to represent the largest cause
  of mortality in the world and claims over 6
  million lives every year (Abdullaev et al.,
  2000).
• An extremely promising strategy for cancer
  prevention today is chemoprevention.

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• Plants, vegetables and herbs used in the folk and
  traditional medicine have been accepted currently
  as one of the main sources of cancer
  chemoprevention drug discovery and development
  (Abdullaev, 2001).
• Antioxidants play an important role in inhibiting
  and scavenging radicals, thus providing
  protection to humans against infection and
  degenerative diseases (Hegazi Abd El Hady, 2009
  and Hegazi et al., 2014).

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• There is a growing interest in the
  pharmacological evaluation of various natural
  products used in traditional medicine.
• Flavonoids, terpenoids, and steroids have
  received considerable attention in recent years
  due to their diverse pharmacological properties
  including antioxidant and antitumor activity
  (DeFeudis et al., 2003 and Takeoka Dao, 2003).

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• From this viewpoint the present study was carried
  out to
• evaluate the antitumor activity,
• immunoglobulin levels,
• phagocytic activity
• lymphocyte transformations
• delayed hypersensitivity
• of coriander honey against Ehrlich ascites
  carcinoma (EAC) in Swiss albino mice.

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• Material and Methods

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Coriander honey

• Coriander honey was purchased from local market
  in Egypt.
• Sterile distilled water was used to dilute honey
  immediately before administration by a stomach
  tube.
• Honey was used in a final concentration of 500
  mg/kg/mouse.

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Animals

• Studies were carried out using a total of 210
  male Swiss albino mice weighting 22- 25 g
  obtained from Animal House of National Research
  Center, Giza, Egypt.

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Ehrlich ascites carcinoma

• Ehrlich ascites carcinoma (EAC) cells were
  obtained from Cancer Biology Section, National
  Cancer Institute, Cairo, Egypt. The Ehrlich tumor
  line was maintained, till the time of the
  experiment in female Swiss albino mice by serial
  intraperitoneal passage of 2106 cells/mouse at
  7-10 days intervals (Hegazi et al., 2014).

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Standard anticancer drug

• 5-Fluorouracil (Calbiochem, USA) was used as a
  standard anticancer drug. 5-Fluorouracil was
  injected intraperitoneally to mice at a dose of
  20 mg/kg body weight (Karthigayan et al., 2007
  and Balamurugan et al., 2010).

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Experimental design

• A total of 210 male Swiss albino mice were
  divided into 6 groups (n35) with an average
  weight 22-25 gm each as follows 

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Groups Group Name Treatment Treatment Treatment Treatment
Groups Group Name Saline Coriander honey EAC 5-floururacil
1 Normal control - - -
2 Coriander control - - -
3 5-flurouracil control - - -
4 EAC control - -
5 Coriander EAC - -
6 5-flurouracil EAC - -
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• At the second day post inoculation with Ehrlich
  carcinoma 0.1 ml of bovine serum albumin was
  inoculated intraperitoneally in each mice in all
  groups as priming for the specific antigen used
  in the skin test.
• Blood and serum samples were collected from 5
  mice from each group at 7, 14 and 21 days
  intervals post inoculation with Ehrlich effusive
  carcinoma cells.
• Blood samples were subjected to determine
  lymphocyte transformation as Hegazi et al. (1981)
  (1985)
• phagocytic activity as Hegazi et al. (1995).
• While the sera sample were used for the
  detection of different Immunoglobulin levels
  IgG, IgA and IgM (Deverill, 1978).

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• After 14 days of the experiment 5 mice from each
  group were subjected to 18 h fasting before
  sacrificing.
• Anti- tumor effect of coriander honey was
  assessed by
• observation of changes in body weight,
• ascetics volume,
• viable and nonviable tumor cell count.

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• The remaining animals in each group were kept
• to check the mean survival time (MST) of the
  tumor bearing hosts
• and increase in life span percentage (ILS ).
• MST of each group containing five mice were
  monitored by recording the mortality daily for 7
  weeks and ILS was calculated using the
  following equation MST (Day of first death
  Day of last death)/2. ILS (Mean survival
  time of treated group/mean survival time of
  control group) -1 X 100 (Mazumder et al., 1997
  and Gupta et al., 2000).

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• Blood and serum samples were collected from 5
  mice from each group at 7, 14 and 21 days
  intervals post inoculation with Ehrlich carcinoma
  cells.
• Blood samples were subjected to determine
• blood lymphocytic count,
• lymphocyte transformation
• The phagocytic activity
• While the sera sample were used for detection of
  different Immunoglobulin levels by quantitative
  determination of IgG, IgA and IgM (Deverill,
  1978).

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• At 72 hours before the end of the experiment a
  0.1-ml of bovine serum albumin was inoculated in
  the left footpad of the mice where the right foot
  pad was inoculated with saline.
• Skin thickness was measured, 24 hours and 72
  hours post inoculation to evaluate the delayed
  type hypersensitivity as Cheville and Beard
  (1972) Hegazi (1981) and Hegazi et al. (1985).
  

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Results
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Influence of honey in mice bearing Ehrlich
carcinoma on immune status
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Influence of honey in mice bearing Ehrlich
carcinoma on immune status
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Influence of honey in mice bearing Ehrlich
carcinoma on immune status
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Influence of honey in mice bearing Ehrlich
carcinoma on immune status
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Influence of honey in mice bearing Ehrlich
carcinoma on immune status
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Influence of honey in mice bearing Ehrlich
carcinoma on immune status
Influence of honey in mice bearing Ehrlich
carcinoma on immune status
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Effect of coriander honey on hematological
parameters in EAC bearing mice.
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Effect of coriander honey on IgM level in mice-
bearing Ehrlich carcinoma 
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Effect of coriander honey on IgG level in mice-
bearing Ehrlich carcinoma 
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Effect of coriander honey on IgA level in mice-
bearing Ehrlich carcinoma 
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Effect of coriander honey on phagocytic activity
level in mice- bearing Ehrlich carcinoma 
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Effect of coriander honey on Lymphocyte
transformation (stimulation index using PHA)
level in mice- bearing Ehrlich carcinoma 
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Effect of coriander honey on Delayed
hypersensitivity (skin index / mm thickness) in
mice- bearing Ehrlich carcinoma 
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Influence of honey in mice bearing Ehrlich
carcinoma on immune status
Thank you for your attention

• Ahmed Hegazi
• National Research Center Dokki, Giza, Egypt

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• Prof. Dr. Ahmed Hegazi
• Professor of Microbiology and ImmunologyNational
  Research Center, Dokki, Giza, Egypt
• President of Egyptian Environmental Society for
  uses and production of bee productsSecretary of
  Egyptian Society of ApitherapySecretary General
  of African  Federation of Apiculture
  AssociationsMember of Apitherapy Commission ,
  APIMONDIAE mail ahmed_at_ahmedhegazi.com and
• ahmedhegazi128_at_gmail.com
• www.ahmedhegazi.com
• Tel       [phone] and 20238860682
  Fax   [phone] and 202 33370931
  GMS 201001440063