2013 Laboratory Safety Training Emergency and Safety Procedures Training VA WNY Healthcare System

1
2013 Laboratory Safety Training Emergency
and SafetyProcedures TrainingVA WNY Healthcare
System
2
Why Have Safety Training?

• Accidents can occur when chemicals are improperly
  transported
• Please read learn why we have safety training

Published on 2007-02-27, Page C2Lexington
Herald-Leader (KY)
3
Why Have Safety Training?

• Accidents can occur when chemicals are improperly
  combined
• Please read learn why we have safety training

Published on 2006-11-04, Page B1Lexington
Herald-Leader (KY)
4
Why Have Safety Training?

• Accidents can be deadly occur when chemicals
  are improperlyhandled
• Please read learn why we havesafety training

Published on 2009-03-01, Page A1Los Angeles
Times (CA)
5
Lab SpecificSafety Training

• The Lab supervisor/PI
• is required to provide
• lab specific safety
• training to staff working in theirin their
  lab(s)
• The following training is to supplement the above
  mentioned training give you an overview of
  emergency and safety procedures

6
Emergency and Safety Procedures Poster

• Found on each research lab door
• Quick reference in caseof an emergency or an
  issue concerning safety

Click on Documentto Open/Print
7
Fire Safety
8
Fire Remember R.A.C.E.
R Rescue
Rescue people in the immediate area
A Activate
Activate by pulling fire Alarm call 4500
C Confine
Confine fire by Closing doors
E Evacuate
Evacuate the floor, Extinguish if a small fire
9
Fire Remember R.A.C.E.

• Fire alarm pull stations are located by each
  stair well
• Every VA phone has listedto report a fire
  (3300) other emergency s (e.g., VA Police,
  Code Zero)
• Evacuation routes are posted inthe hallways.

10
Fire Remember R.A.C.E.

• An announcement will be madeover the PA system
  notifying all personnel of the location of the
  fire
• Code Red location of the fire
• Area fire alarms withflashing lights audible
  alarmswill be activated
• Listen for both in case there is a fire in the
  research area.

11
Fire Extinguishers

• Fire Extinguishers
• Located in some labs
• Do not obstruct or concealfire extinguishers
• Located in hallwaysthroughout Research
• Know where your nearestfire extinguisher is

12
Fire Extinguishers

• When using remember P.A.S.S.
• P Pull the pin
• A Aim at base of fire about 8-10ft away
• S Squeeze the trigger
• S Sweep side to side
• How to Use instructions can be found on
  thefire extinguisher label
• Use only if fire is small you have had training
  on its use

13
Fire Safety and Prevention

• During a fire
• Turn off oxygen, gas, andelectrical equipment in
  the affected area
• Use the stairs Never use the elevators
• Sprinkler heads should befree from obstructions
• Maintain a minimum of 18" between sprinkler
  deflectors along a horizontal plane throughout
  the lab/room

14
Bunsen Burners

• Bunsen Burners/open flame devices should be
  carefully used only when absolutely necessary.
• Induction heaters micro burners are
  alternatives to Bunsen Burners.
• Biosafety Cabinets - open flames are NOT
  recommended. They can damage the HEPA filter
  cabinet.

15
Injury
16
Injury

• Major Go to VA EmergencyDept (ED) if able.
• Minor Notify supervisor and go to VA ED, VA
  Employee Health (8804)
• Code Zero (cardiac/respiratory arrest) Call
  2222 and start CPR

17
HazardousSpills
18
Do Not Clean Up Hazardous Spills.-Isolate the
Spill.-Call for Help!
19
Hazardous Spill Response

• Please remember
• Do not attempt to clean up any hazardous spill
  yourself !
• Notify staff in the immediate area and the
  appropriate safety staff
• Contain spill if possible
• Biological or Chemical Evacuate lab
• Radioactive Stay at lab doorway
• Wait for instructions

20
Biological or Chemical Spill

• Remove any contaminatedclothing or personal
  protective equipment (PPE)
• Use emergency shower or eyewash
• Contain spill if possible
• Evacuate lab, close and lock door
• Call Research Office (x6528)or VA Police (2600)
  after hours
• Remain near lab for instructions

21
Biological or Chemical Spill

• Eyewash stations located in every lab
• Remember to test them weekly
• Document each test
• Report problemsto ResearchOffice (x6528)

22
Biological or Chemical Spill

• Contain spill if it can be done safely
• Spill kits located in hallways on each floor, at
  each end of the building.
• Various spill kits for biologicals,acids,
  caustic (bases),formaldehyde
  solvents(flammables) plus general sorbents
  (pads, socks)

23
Radioactive Spill

• Remove any contaminated clothing PPE. Wash any
  contaminated skin with warm soapy water.
• Contain and/or shield spill if possible. Stay at
  lab door until monitored for contamination.
• Notify staff in the area and the Radiation Safety
  Officer (RSO)x5226 or s posted on door
• below radioactive sign.

24
Radioactive Spill

• Follow RSOs instructions
• Radioactive spill kits located in hot labs B101-1
  and B103-4 along with sorbents
• Spare survey meter located in hot lab, B101-1.

25
Hazardous Spill Response

• Please remember (one more time)
• Do not attempt to clean up any hazardous spill
  yourself!
• Notify staff in the immediate area and the
  appropriate safety staff
• Contain spill if possible
• Biological or Chemical Evacuate lab
• Radioactive Stay at lab doorway
• Wait for instructions

26
Security
27
Lab Security

• Report any suspicious individuals immediately to
  the VA Police (2600)
• Report any lost, stolen, or found VA Research
  keys, or any failures of the Research security
  doors immediately to the Research Office (6528)

28
Lab Security

• Report any lost, missing,potentially dangerous,
  or concerns about hazardous materials or
  radioactive materials immediately to the Research
  Office (x6528), Lab Safety Officer
• (LSO) (x6538) or the RSO
• (x5226)

Picric acid
29
Lab Security

• No tailgating(one swipe card one person in)

30
Lab Security

• Unauthorized visitorsor service
  personnelrequesting entrance to
• Research areas must be sent to the Research
  Office (Building 20, room 129) on the 1st floor
  for assistance
• Authorized lab visitors must sign in out on log
  sheet just inside security door must be
  escorted by lab staff

31
Biosecurity

• Report any missing biological material
• Animal workers are required to complete CITI
  Biosecurity training.
• All new lab employees are required to complete VA
  ORD Biosecurity Training
• Its available on CITI website
  https//www.citiprogram.org/default.asp.

32
Hazardous Waste Disposal
33
Waste Disposal Procedures

• Biological Waste
• Pathological (tissue)
• Red bag waste
• Put in red incineration barrel in RM 203
• Non-Tissue
• Red bag waste
• Put in red bags in lab for EMS disposal
• VMU
• Use red sharps containers in procedure rooms
• All tissue disposal in B114/B117 cold room

34
Waste Disposal Procedures

• Chemical Waste
• Collect waste in appropriatelylabeled container
• Complete and submit Request for a VA Research
  Chemical Pickup electronic form or contact GEMS
  Coordinator at x6018
• Questions can be directedto Laboratory Safety
  Officer
• (x6538)

35
Waste Disposal Procedures

• Radioactive Waste
• Collect in appropriatelabeled shielded(i.e.,
  if needed) container
• Separate containers for
• Type (e.g., dry, liquid, scintillation fluid)
• Nuclide (e.g., H-3, I-125, P-32)
• Call RSO (x5226) for
• instructions

36
Waste Disposal Procedures

• Sharps
• Needles, scalpels, etc.
• Do not cap needles
• Put in sharps container in lab.
• Uncontaminated
• Glassware
• Put in sharps container in lab for
• bi-weekly pickup

37
GEMSGreen EnvironmentalManagement Systems
WNY GEMS Coordinator 716-862-6018
38
GEMS

• What is GEMS?
• The VHA GEMS (Green Environmental Management
  Systems) is a formal system for integrating the
  environmental footprint into the overall
  management of the organization. Required by
  presidential Executive Order 13148, thegoal of
  GEMS is to achieve continual improvement in
  environmental protection.

WNY GEMS Coordinator 716-862-6018
39
GEMS

• In Research
• Properly collect and dispose of biological waste,
  chemicals, radioactive materials, etc.
• Recycle (e.g., paper, batteries, cardboard)
• Replace mercury containing items if
  possible(e.g., thermometers) withnon-mercury
  based items

40
LaboratorySafety
41
General Safety Rules

• No children in the Research labs
• No eating, drinking, or storing of food/drinks in
  labs
• No smoking in labs or the hospital
• No applying cosmetics in labs

42
General Safety Rules

• While working in the lab
• Do not wear sandals
• Do not wear shorts
• Do not wear short skirts
• Do not wear other similar clothes

43
General Safety Rules

• As appropriate, wear
• Lab Coat
• Gloves
• Any other personal protective equipment (PPE)
• Safety glasses/goggles
• Face shield

44
General Safety Rules

• Remove gloves
• Before using phone or computer
• Remove lab coat glovesand wash hands
• Before leaving the lab,unless transporting
  itemsthat require them
• As posted on the inside of lab door

45
General Safety Rules

• Transporting
• Use secondary containment when transporting
  biologicals, chemicals and/or radioactive
  materials
• Eye Wash Station
• As noted earlier, test weekly
• Document testing report any problems (x6538)

46
ChemicalSafety
47
Chemical Safety

• Chemical Inventory
• Each lab(s) must update their chemical inventory
  at least semi-annually and submit it to the
  Research Office
• All chemicals brought into the VA
• (VA ordered as well as those coming over from
  UB) must be delivered to/ checked in by the
  Research Office.

48
Chemical Safety

• Material Data Safety Sheets (MSDS)
• Each lab(s) must have a MSDS binder
• For every chemical in the lab(s), there must be a
  MSDS in this binder

49
Chemical Safety

• Material Data Safety Sheets (MSDS)
• Contains important infoabout the chemical
• Product/company information
• Hazard identification
• First aid measures
• Handling and storage
• Etc. (see attached)
• Please review article on ANSI standardized (
  optional) MSDS format

Click on Documentto Open/Print
50
Chemical Safety

• Chemical Hazard Identification
• Most chemicals have some safety/hazard info
  on the label

PPE Use/Storage
NFPACode
Click on Documentto Open/Print
51
Chemical Safety

• Chemical Hazard
• Identification
• Globally Harmonized
• System (GHS) of
• Classification and
• Labeling of Chemicals

Click on Documentto Open/Print
52
Chemical Safety

• Chemical Labeling
• Every container withany chemical must
  beproperly labeled plus have
• Lab name
• Date received
• Date opened if applicable
• If a chemical is transferred into another
  container, this container must include the
  chemical name along with thelab name and date

53
Chemical Safety

• Proper Chemical Storage
• Separate Flammables, Acids, Bases, Oxidizers, and
  Reactive Chemicals
• Dont store acids/bases above eye level
• Some acids must be storedseparately from other
  acids
• Acetic Acid cabinet in core lab
• Perchloric Acid cabinet in core lab

54
Chemical Safety

• Proper Chemical Storage
• Acetic acid

WARNING Acetic acid (ethanoic acid) when
concentrated is flammable therefore, bulk
concentrated acetic acid MUST be stored in the
flammable cabinet in C326 (core lab) that is
labeled "ACETIC ACID ONLY.

55
Chemical Safety

• Proper Chemical Storage
• Perchloric acid

WARNING Perchloric acid (hydroxidotrioxidochlorin
e) is a strong oxidizer and when in the anhydrous
and monohydrated form, it can be explosive


56
Chemical Safety

• Proper Chemical Storage
• Picric acid

WARNING Picric acid (trinitrophenol) can be
explosive if dry but can be stored safely when
wet (e.g., water content is above 10). DO NOT
OPEN if the contents appears to be dry or
deposits of crystalline solid can be seen inside
the container (e.g., around the container lid)
and immediately notify the VA Laboratory Safety
Officer at x6538 and the VA Safety Office at
x8826.

57
Chemical Safety

• Proper Chemical Storage
• Hazardous peroxide forming chemicals
• Limited shelf life 3-12 months

WARNING
Click on Documentto Open/Print
58
Chemical Safety

• Chemical Usage
• Know the hazardous properties of the chemicals
  you are using!
• Know the hazardous properties of the chemicals
  you are using!
• Know the hazardous properties of the chemicals
  you are using!
• Know the hazardous properties of the chemicals
  you are using!

59
Gas Cylinder Safety

• Only transport usingspecial transport carts
• Secure cylinder to prevent it falling over
• Regulator
• Use proper regulator
• Do not pick up cylinder by the regulator
• Do not lean over regulator when adjusting
• Install safety cap when not in use
• Know the gass hazardous properties

60
Personnel Protective Equipment (PPE)
61
Personnel Protective Equipment

• Specific protection for the hazard to which you
  may be exposed
• Lab coat, gloves, safety glasses while working in
  the lab
• Respiratory protection mayalso be required
• Fume hoods
• Biosafety cabinets
• MINIMIZE SKIN EXPOSURE!!

62
Gloves Chemical Resistant

• Nitrile
• WEAR

• Latex

Wear NITRILE gloves for most applications
(exceptions tissue culture, use of buffers and
aqueous media, etc.)
63
Liquid Nitrogen (LqN2) Use

• Wear appropriate PPE
• Face shield
• Loose-fittingCryogenic gloves
• Cryogenic apron
• Wear appropriate clothing
• Lab coat, cuff-less pants shoesmade of
  non-absorbent material

64
Liquid Nitrogen (LqN2) Use

• Before your first use, contact the Laboratory
  Safety Officer (x6538)for liquid nitrogen
  training
• Store use in a well-ventilated area
• Never use in small closed area/cold room
• Use only LN2 designed containers
• Caution glass lined containers may shatter
• Recommend shatterproof HDPE containers
• Never place in a sealed container!!

65
Fume Hoods

• Do not store chemicals or equipment in fume
  hoods
• Use appropriate PPE
• Use with sash in proper position
• Check for properairflow before using(e.g.,
  tissue on sash alarm)
• Report any diminishedairflow to Research Office
  (x6528)

66
Biosafety
67
Biosafety

• VA Research is conducted at Biosafety Level 1
  (BSL-1) or BSL-2.
• No BSL-3 or BSL-4 studies or agents are permitted
  in the VA.

68
BIOSAFETY LEVEL-2

• BSL-2 agents pose moderate hazards to personnel
  and the environment.
• The primary hazards to personnel working with
  BSL-2 agents relate to accidental percutaneous
  or mucus membrane exposures, or ingestion of
  infectious materials.

69
BIOSAFETY LEVEL-2

• Follow the criteria in the 5th Edition of the
  BMBL (on CDC website) for working safely at
  BSL-2.
• Each lab procedure needs to undergo risk
  assessment and include the proper use of PPE and
  good microbiological techniques.

70
Biological Safety Cabinets

• Follow procedures in the 5th Edition of the BMBL
  (on CDC website) for the proper use of a BSC.
• Use proper PPE.
• Disinfect all biohazardousmaterial after use.
• Make sure the pipetters are clean and in proper
  working order.
• Use no open flames (e.g., No Bunsen Burners)

71
Select Agents
72
Select Agents

• Select Agents are dangerous biological agents
  whose possession and use is highly regulated
• Select agents are NOT allowedin this VA facility
• A current list of Select Agentsis on the
  National Select Agent
• Registry web site!

73
Radiation Safety
74
Radiation Safety

• Radioactive materials can onlybe used/stored in
  rooms thatare labeled for radioactive use
• Only staff who have completedrecent radiation
  safety training can use radioactive materials
  here at this VA
• Contact the RSO at ext 5226to obtain this
  training

75
Environment of Care
76
Environment of Care (EOC)

• EOC monitors the physical facilitiesin Research
  Service
• Report to Research Office (x6528) any
• Lighting malfunction
• Stained ceiling tiles
• Water/air leaks
• Plumbing issues
• Cleaning issues
• Etc.

77
Emergencyand SafetyProcedures
78
Emergency and Safety Procedures

• The Emergency and Safety Procedures Poster and
  this training give you some general guidance but
  it does not cover every issue
• Also available
• Safety Manuals
• Additional Training
• Contacts

79
Safety Manuals

• Each lab(s) should have
• Research Safety Manual (on line)
• Medical Center Environment of Care Management
  Plans (red cover)
• Laboratory Biosafety Manual (orange cover)
• Radiation Safety Records binder(if authorized
  for radioactive materials)
• Posted in hallways
• Emergency OperationProcedures

80
Safety Manuals

• These manuals contain detailed emergency and
  safety procedures that were covered in this
  training plus other issues like
• Severe weather
• Bomb threats
• Utility failures
• Etc.
• Please review what is contained in each of these
  safety manuals.

81
Safety Training

• Supervisor (PI) will provide lab specific safety
  training
• Annual training required
• Lab safety
• Radiation safety(if authorized to
  useradioactive materials)
• Additional training as needed

82
Questions?
If you have any questions about this training, or
questions or concerns about any safety issue in
your lab, please contact your supervisor or any
of the following safety contacts.
83
Safety ContactsKnow how to contact your
supervisor!

• Lab supervisor/PI ???
• Office ???
• Phone ???
• E-mail ???
• (This will be as individual as you are!)

84
Safety Contacts

• Laboratory Safety Officer (LSO)
• Dr. Steven J. Fliesler
• Office Room 202, Building 20
• Phone [phone] or -6538
• Steven.Fliesler_at_va.gov

85
Safety Contacts

• Industrial Hygiene and Safety Manager
• Heidi Reisman
• Office Safety Office
• Phone [phone]
• Heidi.Reisman_at_va.gov

86
Safety Contacts

• Radiation Safety Officer
• Phil Cunningham, Health Physicist
• Office (4C Nuclear Medicine)
• Phone [phone] x5226
• Philip.Cunningham_at_va.gov

87
Safety Contacts

• GEMS Coordinator
• Donald A. Johnson, Environmental Engineer
• Office Safety Office
• Phone [phone]
• Fax [phone]
• Donald.Johnson_at_va.gov

88

• This required training should have given you
• Basic information concerningemergency and safety
  proceduresin Research
• References where to get more detailed information
  concerning emergency and safety procedures in
  Research

Please use this to make a safer work environment
in your lab. Thank you.
89
Required Training Credit

• Complete the training creditform and return it
  to theResearch Office (Room 129) for 1 hour of
  training credit
• You can also get a copy of thistraining credit
  form and/or the presentation with attachments
  through the Research Office

Click on Icon to Open/Complete/Print