University of Nottingham Medical School

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University of Nottingham Medical School Neuronal
Networks Laboratory
Using Plexon at Nottingham Sensory Gating,
Anxiety, Seizures, and Pain Dr Rob
Mason School of Biomedical Sciences University
of Nottingham Medical School

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• Talk Format
• Brief overview of what we have been studying
  using Plexon system(s) at Nottingham
• Indicate on route some hardware developments
• signal processing / analysis approaches we use
• schizophrenia sensory gating
• epilepsy
• anxiety
• pain

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• Multiple Electrode Recordings in vivo
• simultaneous multichannel spike field potential
  recording
• 64-channel MAP system
• 32-channel MAP system with CinePlex for
  behavioural studies
• 64-channel Recorder system
• 16-channel Recorder system
• used MAP Recorder for in vitro MEA studies
  with brain slices neuronal cultures

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LAB TEAM epilepsy Ben Coomber Dr Mike
ODonoghue Clare Roe schizophrenia Dr Jill
Suckling Prof CA Marsden Dr Dissanayake
anxiety Dr Carl Stevenson Prof CA
Marsden pain Dr Steve Elmes Dr V Chapman
rodent USVs Beth Tunstall Dr S Beckett data
analysis Margarita Zachariou Dr S Coombes / Dr M
Owen Mathematics Dept Dr David
Halliday (University of York) Prof D Auer
(fMRI / phMRI)
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• Dual site recordings
• VTA - mPFC
• hippocampus - mPFC
• amygdala - mPFC
• mPFC - mPFC
• Systemic pharmacological manipulation
• Local pharmacological manipulation
• injec-trode
• integrated microiontophoresis with recording
  array
• Electrical stimulation
• Independent electrode array microdrive
• Anaesthetised awake-behaving preparations

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University of Nottingham Medical School Neuronal
Networks Laboratory

• Project 1 Schizophrenia
• auditory-evoked sensory gating
• effects of PCP / ketamine - model
• effects of social isolation - model

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Schizophrenia - VTA/mPFCx dual-site recordings
Prussian Blue reaction - locate recording site(s)
- histology
medial prefrontal cortex - mPFC
ventral tegmental area - VTA
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Dual site recording pre-frontal cortical / VTA
recording multiple single-units LFPs

• Matlab scripts for off-line characterisation
  unit spike waveforms LFPs

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Sensory gating in hippocampus A model for
schizophrenia ?

• Sensory gating mechanism(s) by which irrelevant
  sensory information is filtered enables
  efficient information processing
• Auditory Conditioning-Test paradigm measures
  reduction in auditory-evoked response produced by
  Test stimulus following a Conditioning stimulus
• Stimuli 3kHz sine-wave / 10ms duration /
  presented 500ms apart / 80-90dB
• human P50 wave rat N40 component
• Gating absent in - schizophrenic patients (
  near family members)
• - normal human volunteers given PCP /
  amphetamine
• - rats given PCP / amphetamine

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Hippocampal CA3 - auditory-evoked unit LFP
activity
event
tone
Averaged LFP
T/C ratio 50
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University of Nottingham Medical School Neuronal
Networks Laboratory

• Project 2 Epilepsy
• kainate-induced epileptiform activity TLE
• functional network interactions - hippocampus ?
  mPFC
• electrical stimulation perforant pathway
• role of endocannibnoid system CB1R pharmacology

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Kainate-induced epileptiform activity dual
recording hippocampus mPFC
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Hippocampal-mPFC functional connectivity Burst
analysis Synchrony index - Cross-correlation
coherence - PDC
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University of Nottingham Medical School Neuronal
Networks Laboratory

• Project 3
• Nociception pain management
• dual spinal cord / supraspinal recording
• Role of endocannabinoid system in normal
  physiology and pain (e.g. neuropathic) states

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Mechanically-evoked response in somatosensory
thalamus (VPM)
innocuous (7g) stimulation
noxious (65g) stimulation
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University of Nottingham Medical School Neuronal
Networks Laboratory

• Project 4 Affective state
• Cortico-limibic network interactions
• anxiety
• effects of maternal separation
• pharmacologically-induced (e.g. FG-7142)
  anxiety
• behavioural sequalae
• rodent ultrasound vocalisations

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Combined unit / LFP with USV / behavioural
recording
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Behavioural Electrophysiology II Circular arena
recording using CinePlex
Movie - rat HopScotch 8-microwire array in nuc.
accumbens - 6 weeks post implant
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University of Nottingham Medical School Neuronal
Networks Laboratory
Thats all folks

Lab web site www.nottingham.ac.uk/neuronal-network
s
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